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seedlings大野 豊; 中曽根 光*
Plant Signaling & Behavior (Internet), 8(10), p.e25986_1 - e25986_4, 2013/10
SMALL ACIDIC PROTEIN 1 (SMAP1) functions upstream of the degradation of AUX/IAA-proteins in the response to 2,4-dichlorophenoxyacetic acid and physically interacts with the COP9 SIGNALOSOME (CSN). Also, its function is linked to RELATED TO UBIQUITIN (RUB) modification. To further investigate the relationship between SMAP1 and the RUB modification system, we examined the effect of MLN4924, an inhibitor of RUB/NEDD8-activating E1 enzyme, on the growth of 
. We found that the 
mutants, which lack SMAP1, are more sensitive to MLN4924 than wild type and that SMAP1 is responsible for this hypersensitivity. This new evidence supports our previous speculation that SMAP1 acts in Cullin-RING ubiquitin E3 ligase regulated signaling processes 
its interaction with components associated with the RUB modification system.
mutant in a salt-tolerant accession revealed an importance of salt acclimation ability in plant salt tolerance有賀 裕剛*; 香取 拓*; 吉原 亮平*; 長谷 純宏; 野澤 樹; 鳴海 一成; 井内 聖*; 小林 正智*; 手塚 健二*; 坂田 洋一*; et al.
Plant Signaling & Behavior (Internet), 8(7), p.e24779_1 - e24779_5, 2013/07
シロイヌナズナの354系統の中には、NaCl濃度を0mMから225mmに急激に変化させる試験において、対照となるCol-0系統に比べて高い耐塩性を示す系統がある。また、Zu-Oを含む数系統では、中程度の塩ストレスに暴露された後に高い耐塩性が誘導される。したがって、シロイヌナズナは塩ショックに対する耐性と塩馴化による耐性の少なくとも2種類の耐性機構を有すると思われる。シロイヌナズナの耐塩性系統の耐性メカニズムを明らかにするため、われわれはイオンビームで変異処理したZu-0の実生から塩感受性の変異体を単離した。この変異体は、SOS1遺伝子の1塩基欠失によって塩ショックストレス下で著しい生育抑制を示し、Col-0よりも塩に感受性である。しかし、この変異体はZu-0と同様に、100mMのNaClに7日間暴露した後に750mMのソルビトールに20日間暴露した後も生育することができるが、Col-0は同条件において明らかな白化を示した。したがって、塩馴化に必要な遺伝子は塩ショック耐性に必要な遺伝子とは異なり、塩や浸透圧耐性の獲得に重要な役割を果たしていると考えられる。
, AtRev1 and AtPol
in 
-ray-induced mutagenesis中川 繭*; 高橋 真哉*; 鳴海 一成; 坂本 綾子
Plant Signaling & Behavior (Internet), 6(5), p.728 - 731, 2011/05
Ionizing radiations have been applied to plants as useful mutagens, but its molecular mechanism(s) for mutagenesis is less understood. The AtPol, AtRev1 and AtPol are Arabidopsis translesion synthesis (TLS)-type polymerases involved in the UV-induced mutagenesis. To investigate a role(s) of TLS-type DNA polymerases in radiation-induced mutagenesis, we analyzed the mutation frequency in AtPol-, AtRev1- or AtPol-knockout plants, 
, 
and 
, respectively. The mutation frequency in was little changed, whereas the frequency in greatly decreased and in slightly increased compared to that of wild type. Abasic (apurinic/apyrimidinic; AP) site, induced by radiations or generated during DNA repair process, leads to incorporation of any kinds of nucleotides at the opposite strand. The 7,8-dihydro-8-oxo-2'-deoxyguanosine (8-oxo-dG), induced by radiation through the formation of reactive oxygen species, makes pairs with cytosine and adenines. Therefore, AtRev1 possibly inserts dC opposite AP-site or 8-oxo-dG, which results in G to T transversions.
